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Alpha-amylase For Liquefaction In Ethanol Production

Posted at: Offers to Sell and Export | Posted on: Sun 29 Aug, 2010 7:51 am | Targeted Countries: USA Product Category: Ethanol [23]
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Products Photos CatalogAlpha Amylase Photos Catalog

General description

Boli HS-300 Alpha-Amylase is derived from a non-genetically modified strain of Bacillus

licheniformis utilizing a deep fermentation and refining extraction process. This product is an endoamylase, which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

This product is a very robust alpha-amylase with superior thermal and pH stability. In the ethanol industry, the use of Boli HS-300 results in superior viscosity reduction, allowing high solids mashing and liquefaction, with potential gains in ethanol yields.

Product characterisitcs

Declared Enzyme: Alpha-Amylase

Systematic Name: 1, 4-α-D-glucan glucanohydrolase

Activity: 30000 U/g (minimum)

Appearance: Light to dark brown liquid

Product pH: 5.8 to 6.8

Specific gravity: 1.20 to 1.25 g/ml


This product is active over a wide pH range, depending on the application. For maximum activity, the optimal pH for Boli HS-300 is 6.2 0.2. At a temperature of 25C (77F), the stable pH range for Boli HS-300 is 5.0-10.0. The exact pH optimum depends on many process variables, including temperature, substrate concentration, and time.

Effect of temperature

Optimum temperature of this product for interval liquefaction is greater than 90C. This product liquefies starch substrates promptly at 95C- 97C and is still highly active at 100C. It demonstrates exceptional thermal stability at temperatures up to 105C (maintaining its stability for 5-7 minutes), continuing to liquefy rapidly, efficiently and completely. At temperatures above 105C, the enzyme stability decreases rapidly. The exact temperature optimum will depend on many process variables, including pH, substrate concentration, and time.

Concentration of calcium

The presence of calcium increases the thermal stability of Boli HS-300. This enzyme requires a very low calcium concentration and calcium additions of 50-70 ppm (dry solids basis) are sufficient to ensure enzyme stability.


Copper, titanium, and cobalt ions are moderate inhibitors for Boli HS-300. Aluminum, lead and zinc ions act as strong inhibitors.

Typical usage

In general, first adjust the pH to 6.0-6.4 and then add calcium (50-70 ppm dry solids basis) before adding Boli HS-300. Calcium is not necessarily needed when extremely hard water (> 10 German degrees) is used. After the enzyme is added, heat the slurry to liquefy.


Dosage of this product varies and is dependent on the starch substrate, application, process, substrate concentration, and other factors. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rates is to use 0.028 kg-0.450 kg of Boli HS-300 to liquefy 1MT starch.


This product is packaged in either 30 kg or 1100 kg plastic containers. Customized packaging is also available upon request.


This product should be stored below 25 C (77F) in its original packaging, sealed and unopened, protected from the sun. This enzyme has been formulated for optimal stability. However, enzymes gradually lose activity over time. Extended storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement.


Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to Material Safety Data Sheet.

Company Contact:

JiangsuBoli photo
Contact Name: Edna Yang
Company Name: Jiangsu Boli Bioproducts Co., Ltd.
Email: Email
Tel: +86-(0)523-8207-8001 or +86-151-5221-3005
Fax: +86-(0)523-8207-8002
Street Address: 93 Yangzhou Road,
Jiangyan Economic Development Zone,
Jiangsu 225500, PR China
Website: http://www.bolibio.com
Member name: JiangsuBoli
Country: China-CN China
Member Since: 29 August 2010
Total Leads: 4 JiangsuBoli Import Export Business Leads
Business focus: Enzyme, Alpha-amylase, Fungal Alpha-amylase, Glucoamylase
Verify: Safe Import Export Tips
Product Category: Ethanol [23]

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