Boli HS-300 Alpha-Amylase is derived from a non-genetically modified strain of Bacillus
licheniformis utilizing a deep fermentation and refining extraction process. This product is an endoamylase, which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
This product is a very robust alpha-amylase with superior thermal and pH stability. In the ethanol industry, the use of Boli HS-300 results in superior viscosity reduction, allowing high solids mashing and liquefaction, with potential gains in ethanol yields.
Declared Enzyme: Alpha-Amylase
Systematic Name: 1, 4-α-D-glucan glucanohydrolase
Activity: 30000 U/g (minimum)
Appearance: Light to dark brown liquid
Product pH: 5.8 to 6.8
Specific gravity: 1.20 to 1.25 g/ml
EFFECT OF pH
This product is active over a wide pH range, depending on the application. For maximum activity, the optimal pH for Boli HS-300 is 6.2 ± 0.2. At a temperature of 25ºC (77ºF), the stable pH range for Boli HS-300 is 5.0-10.0. The exact pH optimum depends on many process variables, including temperature, substrate concentration, and time.
Effect of temperature
Optimum temperature of this product for interval liquefaction is greater than 90ºC. This product liquefies starch substrates promptly at 95ºC- 97ºC and is still highly active at 100ºC. It demonstrates exceptional thermal stability at temperatures up to 105ºC (maintaining its stability for 5-7 minutes), continuing to liquefy rapidly, efficiently and completely. At temperatures above 105ºC, the enzyme stability decreases rapidly. The exact temperature optimum will depend on many process variables, including pH, substrate concentration, and time.
Concentration of calcium
The presence of calcium increases the thermal stability of Boli HS-300. This enzyme requires a very low calcium concentration and calcium additions of 50-70 ppm (dry solids basis) are sufficient to ensure enzyme stability.
Copper, titanium, and cobalt ions are moderate inhibitors for Boli HS-300. Aluminum, lead and zinc ions act as strong inhibitors.
In general, first adjust the pH to 6.0-6.4 and then add calcium (50-70 ppm dry solids basis) before adding Boli HS-300. Calcium is not necessarily needed when extremely hard water (> 10 German degrees) is used. After the enzyme is added, heat the slurry to liquefy.
Dosage of this product varies and is dependent on the starch substrate, application, process, substrate concentration, and other factors. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rates is to use 0.028 kg-0.450 kg of Boli HS-300 to liquefy 1MT starch.
This product is packaged in either 30 kg or 1100 kg plastic containers. Customized packaging is also available upon request.
This product should be stored below 25 ºC (77ºF) in its original packaging, sealed and unopened, protected from the sun. This enzyme has been formulated for optimal stability. However, enzymes gradually lose activity over time. Extended storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement.
SAFETY & ENZYME HANDLING
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to Material Safety Data Sheet.
Contact Name: Edna Yang
Company Name: Jiangsu Boli Bioproducts Co., Ltd.
Tel: +86-(0)523-8207-8001 or +86-151-5221-3005
Street Address: 93 Yangzhou Road,
Jiangyan Economic Development Zone,
Jiangsu 225500, PR China
Member name: JiangsuBoli
Member Since: 29 August 2010
Total Leads: 4 JiangsuBoli Import Export Business Leads
Business focus: Enzyme, Alpha-amylase, Fungal Alpha-amylase, Glucoamylase
Verify: Safe Import Export Tips
Product Category: Ethanol