The hog cholera virus test kit is used for the detection of hog cholera virus antibodies in
swine serum; assessment of immunity conditions against cholera virus in the pig farm and epidemiology investigation of the hog cholera.
Hog cholera (HC) is a highly contagious viral disease of swine that occurs in an acute, a subacute, a chronic, or a persistent form. In the acute form, the disease is characterized by high fever, severe depression, multiple superficial and internal hemorrhages, and high morbidity and mortality. In the chronic form, the signs of depression, anorexia, and fever are less severe than in the acute form, and recovery is occasionally seen in mature animals. Transplacental infection with viral strains of low virulence often results in persistently infected piglets, which constitute a major cause of virus dissemination to noninfected farms.
Although minor antigenic variants of hog cholera virus (HCV) have been reported, there is only one serotype. Hog cholera virus is a lipid-enveloped pathogen belonging to the family Flaviviridae, genus Pestivirus. The organism has a close antigenic relationship with the bovine viral diarrhea virus (BVDV) and the border disease virus (BDV), as demonstrated in the immunodiffusion and immunofluorescence tests. The serum neutralization test can, however, differentiate between HCV and BVDV. In a protein-rich environment, HCV is very stable and can survive for months in refrigerated meat and for years in frozen meat. The virus is sensitive to drying (desiccation) and is rapidly inactivated by a pH of less than 3 and greater than 11.
The hosts of HCV are the pig and wild boar.
The test kit is made from the antigen coated microtiter plate, goat-anti-pig IgG-HRP and other reagents. It applies the indirect ELISA principle to test the antibodies against hog cholera virus in pig serum.
The test kit is the antigen coated microtiter plate made through the inactivation, purification and concentration of the hog cholera virus. In the test, add the diluted control serum and sample into the wells, then incubate. If specific hog cholera virus antibodies exist in the sample, it will be bound with the antigens of the microtiter plate, the unbound antibodies and other components will be removed by washing. Next, add enzyme conjugate (anti-pig IgG-HRP) to specifically bind with the compound on the microtiter plate. The unbound conjugate will be removed by washing, add the TMB substrate to the wells, react and generate a blue color. At last, end the reaction by adding stop solution.
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
Street Address: Rm.507, No.2,
Longgang Overseas Venture Park,
Member name: Shenzhenlvshiyuanbiotec
Member Since: 07 March 2008
Total Leads: 69 Shenzhenlvshiyuanbiotec Import Export Business Leads
Business focus: Animal Disease Test Kit, Food Safety Test Kit
Verify: Safe Import Export Tips