This immunoassay kit allows for the
specific measurement of human Complement C3 concentrations in serum and plasma.
Complement C3 is the major plasma proteins of the immune system complement pathways. The synthesis of these proteins is increased in response to inflammation and infection but at a slower rate than for traditional acute phase proteins.
Complement protein C3 is the fourth component to attach in the complement reaction sequence. It is a beta-globulin with a sedimentation coefficient of 5.5 and a molecular weight of 185, 000. C3 is a central molecule in the complement system whose activation is essential for all the important functions performed by this system. C3 can promote phagocytosis during an inflammatory response against pathogens, but unregulated activation of C3 could lead to host cell damage. Low level of serum C3 associates with hypocomplementaemia, primary biliary cirrhosis
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Complement C3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Complement C3 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Complement C3 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Complement C3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Contact Name: Lucia
Company Name: Wuhan Eiaab Science Co., Ltd
Street Address: A1710 Guangguguoji,
East Lake Hi-tech Zone, Wuhan, China
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Member Since: 03 March 2011
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