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Human Complement Fragment 3a Elisa Kit

Posted at: Offers to Sell and Export | Posted on: Thu 02 Jun, 2011 8:31 am |
 
human complement fragment 3a elisa kit
Products Photos CatalogHuman Complement Photos Catalog



E0387h

Elisa kit


96t

Uscnlife&EIAAB

Usd736

Intended use

This immunoassay kit allows for the

in vitro quantitative determination of human C3a concentrations in serum, plasma and other biological fluids.

Introduction

C3a is generated during the activation of the complement system via the classical or alternative pathway. The anaphylatoxin C3a itself is very short lived and in serum is cleaved rapidly to the more stable C3a-desArg. Therefore, quantitation of C3a-desArg allows reliable conclusions about the level of complement activation in a test sample.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of C3a in the samples is then determined by comparing the O.D. of the samples to the standard curve.



Company Contact:

uscnlife photo
Contact Name: Lucia
Company Name: Wuhan Eiaab Science Co., Ltd
Email: Email
Tel: 0086-27-87490292
Fax: 0086-27-87569450
Street Address: A1710 Guangguguoji,
East Lake Hi-tech Zone, Wuhan, China
Website: http://www.eiaab.com
Member name: uscnlife
Country: China-CN China
Member Since: 03 March 2011
Total Leads: 411 uscnlife Import Export Business Leads
Business focus: Uscnlife, Elisa Kit, Eiaab, Diagnostic Kits, Assay Kits, Test Kits, Elisa Kit Company, Elisa Kits Manufacturer
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