This immunoassay kit allows for the
in vitro quantitative determination of human C3a concentrations in serum, plasma and other biological fluids.
C3a is generated during the activation of the complement system via the classical or alternative pathway. The anaphylatoxin C3a itself is very short lived and in serum is cleaved rapidly to the more stable C3a-desArg. Therefore, quantitation of C3a-desArg allows reliable conclusions about the level of complement activation in a test sample.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of C3a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Contact Name: Lucia
Company Name: Wuhan Eiaab Science Co., Ltd
Street Address: A1710 Guangguguoji,
East Lake Hi-tech Zone, Wuhan, China
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Member Since: 03 March 2011
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