This immunoassay kit allows for the in vitro quantitative
determination of human Erythropoietin, EPO concentrations in serum, plasma and other biological fluids.
Erythropoietin (EPO) is A hormone produced by the kidney that promotes the formation of red blood cells in the bone marrow. EPO is a glycoprotein (a protein with a sugar attached to it). Human EPO has a molecular weight of 34, 000. The kidney cells that make EPO are specialized and are sensitive to low oxygen levels in the blood. These cells release EPO when the oxygen level is low in the kidney. EPO then stimulates the bone marrow to produce more red cells and thereby increase the oxygen-carrying capacity of the blood.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to EPO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of EPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Canine Erythropoietin, EPO ELISA Kit
Human Erythropoietin, EPO ELISA Kit
Mouse Erythropoietin, EPO ELISA Kit
Rat Erythropoietin, EPO ELISA Kit
Contact Name: Lucia
Company Name: Wuhan Eiaab Science Co., Ltd
Street Address: A1710 Guangguguoji,
East Lake Hi-tech Zone, Wuhan, China
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Product Category: Erythropoietin