Nitrofuran Aoz Elisa Test Kit

1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AOZ (3-anmino-2-oxazolidinone) in the meat, chicken, fish,shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with AOZ concentration in the sample. This value is compared to the standard curve and the content of the corresponding AOZ is subsequently obtained.

2. Technical specifications
Sensitivity : 0.05ppb
Detection limit:
Tissue and honey—————————--0.1ppb
Shrimp,fish(some interference in shrimp and fish) ——— 0.2ppb
Recovery rate
Shrimp and fish ———————— 90±10%
Honey ,chicken meat /liver ——— 75±15%
Cross-reaction rate:
AOZ ——————100%
AHD —————﹤0.1%
AMOZ —————﹤0.1%
SEM —————﹤0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1ml each): 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb
3） Enzyme conjugate (12ml) …………………….…………red cap
4） Antibody working solution (7ml) ……………..………. .blue cap
5） Substrate A solution (7ml) …………………..…….….. white cap
6） Substrate B solution (7ml) …………………..……….. black cap
7） Stop solution (7ml) ……………………………………. yellow cap
8） 20× concentrated washing buffer (40ml)……..…….. white cap
9） 2× concentrated redissolving solution (50ml)…….. transparent cap
10) 2-Nitrobenzaldehyde(C7H5NO3)( 15.1mg)……………….white cap

_________________
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
Email: Lsybt01@gmail.com
Tel: +86-0755-28938075
Fax: +86-0755-28938800
Street Address: Rm.507, No.2,
Longgang Overseas Venture Park,
Shenzhen,China