Porcine Encephalitis Virus ELISA Test kit
The porcine encephalitis virus ELISA test kit is
used for the detection of the porcine encephalitis virus antibodies in swine serum; assessment the immunity conditions against porcine encephalitis virus in the pig farm; and investigation of the epidemiology of the porcine encephalitis virus.
The test kit is made from the antigen coated microplate, enzyme conjugations(HRP goat-anti-pig IgG), and other reagents. It applies the indirect ELISA principle to test the antibodies against porcine encephalitis virus in pig serum.
The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the porcine encephalitis virus. In the test, the diluted control serum and sample are added, then incubate. If porcine encephalitis Virus specific antibodies exists in the sample, It will be bound with the antigens on the microplate, but the unbound antibodies and other components will be removed by washing. Next, add enzyme conjugations(anti-pig IgG-HRP) to specifically bind with the compound on the microplate. Then the unbound conjugations will be removed by washing. Add the TMB substrate in the well, react with HRP to a blue product. At last, end the reaction by adding stop solution.
Japanese B encephalitis is caused by a small RNA virus, a flavivirus 40 nm in diameter. It has been defined antigenically and by restriction endonuclease techniques (techniques which cut nucleic acid and demonstrate fragments). It is extremely unstable outside the body and is rapidly inactivated by disinfectants, heat and extremes of pH. It grows well in cell culture. Infection results from mosquito bites principally Culex tritaeniorhynchus but other mosquito species may be involved (C. vishnui, India, C. annulirostris, Torres Straits). Wild birds may be a reservoir and infection can be transmitted to man. Viraemia follows infection
The central nervous system may be affected but the major effects result from infection of the testis and the development or orchitis and multiplication in the foetus between 40 and 60 days gestation to cause foetal death. Virus may persist in spleen macrophages and has been demonstrated by RT-PCR (a Polymerase Chain Reaction) during winter when virus cannot be isolated from the pig. Infection may be transmitted in semen. The pig is a reservoir species from which the disease is transmitted to man by mosquito bites.
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