Porcine Parvovirus Infection (PPV) is the most common and important cause of infectious infertility.
Porcine parvovirus is a fairly tough virus that multiplies normally in the intestine of the pig without causing clinical signs. It is world-wide in its distribution. If you test for it in your pig herd it is almost certain it will be present unless your herd is less than 100 sows when it might have died out. It is therefore an infection you have to live with and manage. Whereas most viruses do not survive outside the host for any great period of time PPV is unusual in that it can persist outside the pig for many months and it is resistant to most disinfectants. This perhaps explains why it is so widespread and so difficult to remove from the pig environment.
Porcine Parvovirus ELISA test Kit
The porcine parvovirus ELISA test kit is used for the detection of porcine parvovirus antibodies in swine serum; assessment of immunity conditions against porcine parvovirus in the pig farm; investigation epidemiology of the porcine parvovirus.
2. Brief Introduction
The test kit is composed of the antigen coated microplate, HRP goat-anti-pig IgG, and other reagents; it applies the indirect ELISA principle to test the antibodies against porcine parvovirus in pig serum.
The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the porcine parvovirus. In the test, the diluted control serum and sample are added, then incubate. If porcine parvovirus specific antibodies exist in the sample, it will be bound with the antigens on the microplate, remove the unbound antibodies and other components by washing. Add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. Then the unbound enzyme conjugate (Anti-pig IgG-HRP combination) will be removed by washing. Then add the TMB substrate to the wells, react with HRP to generate a blue color. Stop the reaction by adding HF solution, measure the OD630nm value.
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