Porcine reproductive and respiratory syndrome is a serious swine disease that appeared suddenly in the
midwestern United States and central Europe approximately 14 years ago; the disease has now spread worldwide. In North America and Europe, the syndrome is caused by two genotypes of porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus whose genomes diverge by approximately 40%. My hypothesis, which explains the origin and evolution of the two distinct PRRSV genotypes, is that a mutant of a closely related arterivirus of mice (lactate dehydrogenase-elevating virus) infected wild boars in central Europe. These wild boars functioned as intermediate hosts and spread the virus to North Carolina in imported, infected European wild boars in 1912; the virus then evolved independently on the two continents in the prevalent wild hog populations for approximately 70 years until independently entering the domestic pig population.
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), also known as Blue-Ear Pig Disease (in Chinese, zhū láněr běng), is a virus that causes a disease of pigs, called Porcine Reproductive and Respiratory Syndrome (PRRS). This economically important pandemic disease causes reproductive failure in breeding stock and respiratory tract illness in young pigs. Initially referred to as "mystery swine disease" and "mystery reproductive syndrome, " it was first reported in 1987 in North America (2) and Central Europe (3). The disease costs the United States swine industry around $600 million annually.
PRRSV occurs in most major pig-producing areas throughout the world. The reproductive failure is characterised by abortions, stillbirths, and the birth of weak piglets that often die soon after birth of respiratory disease and secondary infections. Older pigs may demonstrate mild signs of respiratory disease, sometimes complicated by secondary infections. Animals other than pigs do not seem to be affected by PRRSV.
The porcine reprodutive and respiratory syndrome virus (PRRSV) ELISA test kit is used for the detection of PRRSV antibodies in swine serum; assessment of immunity conditions against porcine reprodutive and respiratory syndrome virus in the pig farm; and investigation epidemiology of the PRRSV.
The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the PRRSV. In the test, the diluted control serum and sample are added, then incubate. If porcine PRRSV specific antibodies exist in the sample, it will be bound with the antigens on the microplate, remove the unbound antibodies and other components by washing. Add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. Then the unbound enzyme conjugate (Anti-pig IgG-HRP combination) will be removed by washing. Then add the TMB substrate to the wells, react with HRP to generate a blue color. At last, end the reaction by adding stop solution, read the absorbance value at 630 nm.
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