PRRSV RT-PCR test kit
This test was assessed for sensitivity and application as a rapid diagnostic
tool by comparison with virus isolation and detection of PRRS virus antibody in blood. The RT-PCR test was slightly more sensitive than virus isolation for detection of virus in serum and markedly more sensitive than virus isolation from plasma from experimentally infected pigs.
1 RNA in refrigerated serum sample is extracted by TRIzol, The cDNA synthesis was performed with RNA template and specific primer . To produce fragment of PRRSV, degenerate at high temperature, anneal at low temperature, transcript at mid-temperature, cycle with Taq DNA, amplify million fold through 35 times cycle. Electrophoresize RT-PCR products, dyed by ETBR, generate specific fragments in the ultraviolet lights which can be seen by eyes.
A solution: Positive control 60 ul
B solution: TriZol solution 7ml
C solution: CHCl3 3.5ml
D solution: C3H3O 4ml
E solution: CH3CH2OH 12ml
F solution: DEPC-Deionized water: 500ul
G solution:RT-PCR buffer 22.5ul X11
H solution:sample buffer: 50ul
B, C, D, H, is stored at 4-8 ℃; A, E, F, G, stored at -20 ℃;donít froze repeatedly.
Expiry date:3 months.
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
Street Address: Rm.507, No.2,
Longgang Overseas Venture Park,
Member name: Shenzhenlvshiyuanbiotec
Member Since: 07 March 2008
Total Leads: 69 Shenzhenlvshiyuanbiotec Import Export Business Leads
Business focus: Animal Disease Test Kit, Food Safety Test Kit
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