The pseudorabies ELISA antibody test kit is used for the detection of the pseudorabies antibodies
in swine serum; assessment the immunity conditions against pseudorabies in the pig farm, investigation the epidemiology of the pseudorabies.
The test kit is made from the antigen coated microplate, HRP goat-anti-pig IgG and other reagents. It applies the indirect ELISA principle to test the antibodies against pseudorabies virus in pig serum.
The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the pseudorabies virus. In the test, the diluted control serum and sample are added, then incubate. If pseudorabies specific antibodies exist in the sample, it will be bound with the antigens on the microplate, but the unbound antibodies and other components will be removed by washing. Next adding enzyme conjugations(anti-pig IgG-HRP) to specifically bind with the compound on the microplate. Then the unbound conjugations are removed by washing, add the TMB substrate to the wells, react with HRP to a blue product. At last, end the reaction by adding stop solution.
Reagents of the Test Kit
1 Antigen coated microplate 2 pieces (96 wells)
2 Negative (green lid)/positive (red lid) serum 1 tube each (1ml/tube)
3 Anti-pig IgG-HRP combination(yellow lid) 1 bottle (22ml/bottle)
4 20× concentrated washing solution (white lid) 1 bottle (30ml/bottle)
5 Substrate A (red lid)/B (black lid) 1bottle each (12ml/bottle)
6 Stop solution(blue lid) 1 bottle (12ml/bottle)
7 Sample diluent solution(transparent lid) 1 bottle (50ml/bottle)
Material Required But Not Provided
50μl-, 100μl-, 1000-micropipettes, adjustable.
Constant temperature box or incubator.
Microtiter plate spectrophotometer(630nm)(or ELIASA).
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
Street Address: Rm.507, No.2,
Longgang Overseas Venture Park,
Member name: Shenzhenlvshiyuanbiotec
Member Since: 07 March 2008
Total Leads: 69 Shenzhenlvshiyuanbiotec Import Export Business Leads
Business focus: Animal Disease Test Kit, Food Safety Test Kit
Verify: Safe Import Export Tips