Pseudorabies is a viral disease in swine that is endemic in most parts of the world. It is caused by
porcine herpesvirus 1, which is also called pseudorabies virus (PRV) or suid herpesvirus-1 (SHV-1) and is also known as Aujeszky's disease, and in cattle as mad itch. PRV is considered to be the most economically important viral disease of swine in areas where hog cholera has been eradicated.
Research on PRV in pigs has pioneered animal disease control with genetically modified vaccines. PRV is now extensively studied as a model for basic processes during lytic herpesvirus infection, and for unravelling molecular mechanisms of herpesvirus neurotropism.
Pseudorabies Virus gE Antibody ELISA Test Kit
Pseudorabies virus gE antibody ELISA test kit can be used to detect anti PRV-gE antibody in the serum and distinguish naturally infected from vaccined swine.
The test kit is made from the antigen coated microplate, enzyme conjugate(HRP goat-anti-pig IgG) and other reagents. It applies the indirect ELISA principle to test the antibody against PRV-gE antibodies in the serum.
The test kit is the antigen coated microplate made through the protein expressed by pseudorabies virus gE gene. In the test, the diluted control serum and sample are added, then incubate. If PRV-gE specific antibodies exist in the sample, it will be bound with the antigens of the microplate. Then the unbound antibodies and other components are removed by washing. Next, add IgG-HRP to specifically bind with the compound of antibody and antigen on the microplate. The unbound conjugation will be removed by washing, add the TMB substrate in the well, react with HRP to a blue product. At last, end the reaction by adding stop solution, .
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
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