Swine Foot and Mouth Disease Distinguishing Test Kit
Swine Foot and Mouth Disease Distinguishing
ELISA Test kit is used for detection the non-structural 3ABC-protein of swine foot and mouth disease (FMD-3ABC) antibodies in the serum, differentiation naturally infected from vaccined swine.
The test kit is made from the antigen coated microplate, enzyme conjugation(HRP goat-anti-pig IgG), and other reagents, it applies the indirect ELISA principle to test the antibodies against FMD-3ABC in the serum.
The test kit is the antigens coated microplate made through FMD-3ABC protein. In the test, the diluted control serum and sample are added, then incubate. If FMD-3ABC specific antibodies exist in the sample, it will be bound with the antigens of the microplate but the unbound antibodies and other components will be removed by washing. Next add enzyme conjugation (anti-pig IgG-HRP) to specifically bind with the compound of antibodies and antigens on the microplate. Then the unbound conjugations will be removed by washing, Add the TMB substrate in the well, react with HRP to a blue product. At last, end the reaction by adding stop solution.
Foot-and-mouth disease (FMD) is a severe, highly communicable viral disease of cattle and swine. It also affects sheep, goats, deer, and other cloven-hoofed ruminants. FMD is not recognized as a zoonotic disease.
This country has been free of FMD since 1929, when the last of nine U.S. outbreaks was eradicated.
The disease is characterized by fever and blister-like lesions followed by erosions on the tongue and lips, in the mouth, on the teats, and between the hooves. Many affected animals recover, but the disease leaves them debilitated. It causes severe losses in the production of meat and milk.
Because it spreads widely and rapidly and because it has grave economic as well as clinical consequences, FMD is one of the animal diseases that livestock owners dread most.
The disease is caused by a virus. The virus survives in lymph nodes and bone marrow at neutral pH, but destroyed in muscle when in pH<6.0, i.e., after rigor mortis. The virus can persist in contaminated fodder and the environment for up to one month, depending on the temperature and pH conditions.
There are at least seven separate types and many subtypes of the FMD virus. Immunity to one type does not protect an animal against other types
FMD viruses can spread by animals, people, or materials that bring the virus into physical contact with susceptible animals. An outbreak can occur when:
- people wearing contaminated clothes or footwear or using contaminated equipment pass the virus to susceptible animals
- animals carrying the virus are introduced into susceptible herds
- contaminated facilities are used to hold susceptible animals
- contaminated vehicles are used to move susceptible animals
- raw or improperly cooked garbage containing infected meat or animal products is fed to susceptible animals
- susceptible animals are exposed to materials such as hay, feedstuffs, hides, or biologics contaminated with the virus.
- susceptible animals drink common source contaminated water
- a susceptible cow is inseminated by semen from an infected bull.
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
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