Aflatoxins are secondary metabolites of the fungi species Aspergillus
flavus, parasiticus and nomius. Aflatoxins belong to the strongest natural occurring carcinogenic substances, commonly found in corn, peanuts, cotton seed, human blood and animal feed.Aflatoxin B1 appears nearly in all cases together with Aflatoxin B2, G1 and G2 and it is the analyte with the highest toxic significance.Thin-layer chromatography has been a common method of aflatoxin detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of aflatoxin B1 ELISA kit to analyze aflatoxin B1 residuesis of samples is fast and accurate. Refer to the National Standard GB / T 5009.22-2003.
This kit is a new generation of fungal toxins detection products in application of ELISA technology and the operation time is shorter than 60min, to minimize operating errors and work intensity.
2. Test principle
The test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with aflatoxin antigen. Free aflatoxin in sample solutions and the immobilized antigen compete for aflatoxin enzyme conjugate. Any unbound enzyme conjugate is then removed in a washing step. Substrate/chromogen is added to the wells, bound enzyme conjugate converts the chromogen into a blue product. The addition of the stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorbance is inversely proportional to the aflatoxin concentration in the sample.
This test kit can be used to detect aflatoxin B1 in maize, rice, wheat, beans, peanuts, peanut butter, feed, edible oil and other samples qualitatively and quantitatively .
4. Reagents provided
Each kit contains sufficient materials for 96 measurements (including standard analyses). Each test kit contains:
1 x Microtiter plate (12 strips with 8 removable wells each) coated with capture antigen
6 x Aflatoxin B1 standards, 1 ml each
0 ppb (zero standard), 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
1 x High concentration aflatoxin B1 standards, 1ml 100ppb
1 x Enzyme labeled antibody 6 ml
peroxidase conjugated anti-aflatoxin B1 antibodies
1 x Substrate A 6ml
1 x Substrate B 6ml
1 x Stop solution6ml
1 x Concentrated washing buffer40ml
1 x Concentrated sample diluent40ml
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
Street Address: Rm.507, No.2,
Longgang Overseas Venture Park,
Member name: Shenzhenlvshiyuanbiotec
Member Since: 07 March 2008
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Business focus: Animal Disease Test Kit, Food Safety Test Kit
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