This immunoassay kit allows for the
in vitro quantitative determination of human cGMP concentrations in tissue homogenates and other biological fluids.
Guanosine 3’, 5’-cyclic monophosphate (cGMP) is a multi-functional second messenger molecule. It is generated via two pathways distinguished by the types of Guanylyl Cyclase (GC) that mediate its conversion from guanosine triphosphate (GTP). In the soluble pathway, cGMP is generated via cytoplasmic nitric oxide (NO)-activated GC. GCs in the particulate pathway share some homology with those activated by NO but are transmembrane proteins with extracellular ligand-binding domains. The ligands for a subset of membrane GCs are members of the Natriuretic Peptide (NP) hormone family including Atrial NP hormone, B-type NP hormone, and C-type NP hormone.
cGMP primarily affects cellular activities through four different pathways. These include cGMP-dependent Protein Kinases (PKG/GK), cyclic nucleotide-gated (CNG) channels, cAMP-dependent Protein Kinase (PKA), and Phosphodiesterases.PKGs (PKG I and PKG II) are serine/threonine kinases activated by cGMP.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to cGMP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for cGMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain cGMP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of cGMP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Contact Name: Lucia
Company Name: Wuhan Eiaab Science Co., Ltd
Street Address: A1710 Guangguguoji,
East Lake Hi-tech Zone, Wuhan, China
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