This immunoassay kit allows for the
in vitro quantitative determination of human GNLY concentrations in tissue homogenates and other biological fluids.
Granulysin is a substance released by cytotoxic T cells (CD8) when they are attached to infected body cells. It functions to create holes in the microbe and destroy it. Granulysin is able to induce apoptosis in target cells and also has antimicrobial action.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GNLY. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GNLY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GNLY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of GNLY in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Contact Name: Lucia
Company Name: Wuhan Eiaab Science Co., Ltd
Street Address: A1710 Guangguguoji,
East Lake Hi-tech Zone, Wuhan, China
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